Modern LC-MS/MS proteomics relies heavily on how peptide ions are selected and fragmented inside the mass spectrometer. In shotgun proteomics, thousands of peptides may co-elute within a single LC gradient. Since the instrument cannot fragment ever…
A chimeric spectrum in LC-MS/MS is an MS/MS spectrum that contains fragment ions originating from multiple precursor ions rather than a single peptide. Chimeric spectra occur when more than one ion is simultaneously isolated and fragmented during ta…
In LC-MS/MS-based proteomics, one of the most important analytical goals is accurate peptide sequence identification. Most proteomics workflows rely on database search engines such as: Mascot Sequest MS-GF+ These approaches compare experimental MS/M…